An HIV type 1 subtype A outbreak among injecting drug users in Kazakhstan

被引：27
作者：
Bobkov, AF [1 ]
Kazennova, EV
Sukhanova, AL
Bobkova, MR
Pokrovsky, VV
Zeman, VV
Kovtunenko, NG
Erasilova, IB
机构：
[1] DI Ivanovskii Inst Virol, Moscow 123098, Russia
[2] Fed Ctr Aids, Moscow, Russia
[3] AIDS Ctr Reference Lab, Alma Ata, Kazakhstan
来源：
AIDS RESEARCH AND HUMAN RETROVIRUSES | 2004年 / 20卷 / 10期
关键词：
D O I：
10.1089/aid.2004.20.1134
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Kazakhstan experienced the start of the HIV-1 outbreak among intravenous drug users (IDUs) in 1997. To characterize genetically HIV-1 strains circulating in this country, peripheral blood mononuclear cells (PBMCs) DNA samples (1999-2002) derived from HIV-infected IDUs and their sexual partners in Pavlodar (n = 19), Shymkent (n = 6), and Qaraghandy (n = 18) regions were analyzed by the gag/env heteroduplex mobility assay (HMA). The 366-bp proviral env gene fragments encoding the gp120 C2-V3 region obtained from 16 individuals were sequenced. The results of HMA revealed that all 43 HIV-1 strains studied belonged to gag/env subtype A. The nucleotide sequence analysis showed a marked genetic homogeneity with the mean genetic distance being 3.63 +/- 2.39 (range 0.00-12.13). The mean genetic distance between each sequence within the Kazakhstan set and the East-European IDU subtype A consensus was 2.94 +/- 1.92 (range 0.79-8.48). The data presented thus confirm the spreading of the same IDU subtype A virus in the former Soviet Union.
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收藏
页码：1134 / 1136
页数：3
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