Antioxidant activity of Astragalus polysaccharides and antitumour activity of the polysaccharides and siRNA

被引：171

作者：

Li Rui ^{[1
]}

Chen Wei-chang ^{[1
]}

Wang Wei-peng ^{[2
]}

Tian Wen-yan ^{[1
]}

Zhang Xue-guang ^{[3
]}

机构：

[1] Soochow Univ, Affiliated Hosp 1, Dept Gastroenterol, Key Lab Med & Clin Immunol Jiangsu Prov, Suzhou 215006, Peoples R China

[2] Soochow Univ, Coll Pharm, Suzhou 215007, Peoples R China

[3] Soochow Univ, Inst Med Biotechnol, Suzhou 215007, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 82卷 / 02期

基金：

中国国家自然科学基金;

关键词：

FT-IR; Antioxidant; Antitumour; Astragalus polysaccharides; siRNA; Psilencer1; 0-U6-PI3K-siRNA; DERIVATIVES; ENZYMES; STRESS; RADIX;

D O I：

10.1016/j.carbpol.2010.02.048

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, Astragalus polysaccharides was analysed using high performance liquid chromatography (HPLC) and Fourier transform infrared (FT-IR). Compared with retention time of six standard compounds in HPLC analyses, we found that the polysaccharides were composed of glucose. The absorption bands at 1639 and 1538 cm(-1) were attributed to the stretching vibration of the C-O bond of carboxyl group. The absorption bands at 1035 and 956 cm(-1) suggested that the extract contained pyrene monomer in its structure. The antioxidant activity of Astragalus polysaccharides was evaluated by various antioxidant assays. Astragalus polysaccharides showed strong antioxidant activity in all the tested methods. The in vitro antioxidant capacity of Astragalus polysaccharides was significantly correlated with its content. In addition to the antioxidant activity of the polysaccharides, it still showed strong antitumour activity. Then, CD40 gene siRNA plasmid (Psilencer1. 0-U6-P I3K-siRNA) was constructed. Antitumour activity of si RNA was evaluated. Among these samples, Astragalus polysaccharides exhibited more antitumour potency than siRNA. Mechanism of antitumour activity of Astragalus polysaccharides and si RNA may be explained by decreasing CD40 in cells. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：240 / 244

页数：5

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