Involvement of Auxin and Brassinosteroid in Dwarfism of Autotetraploid Apple (Malus x domestica)

被引：88

作者：

Ma, Yue ^{[1
]}

Xue, Hao ^{[1
]}

Zhang, Lei ^{[1
]}

Zhang, Feng ^{[1
]}

Ou, Chunqing ^{[2
]}

Wang, Feng ^{[3
]}

Zhang, Zhihong ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Hort, 120 Dongling Rd, Shenyang 110866, Peoples R China

[2] Chinese Acad Agr Sci, Res Inst Pomol, Xingcheng 125100, Liaoning, Peoples R China

[3] Shenyang Agr Univ, Coll Plant Protect, 120 Dongling Rd, Shenyang 110866, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

GENE-EXPRESSION; INTERNODE LENGTH; PLANT-GROWTH; ARABIDOPSIS; OVEREXPRESSION; MECHANISMS; BIOSYNTHESIS; CONSEQUENCES; POLYPLOIDY; MUTANT;

D O I：

10.1038/srep26719

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The plant height is an important trait in fruit tree. However, the molecular mechanism on dwarfism is still poorly understood. We found that colchicine-induced autotetraploid apple plants (Malus x domestica) exhibited a dwarf phenotype. The vertical length of cortical parenchyma cells was shorter in autotetraploids than in diploids, by observing paraffin sections. Hormone levels of indoleacetic acid (IAA) and brassinosteroid (BR) were significantly decreased in 3- and 5-year-old autotetraploid plants. Digital gene expression (DGE) analysis showed that the differentially expressed genes were mainly involved in IAA and BR pathways. microRNA390 was significantly upregulated according to microarray analysis. Exogenous application of IAA and BR promoted stem elongation of both apple plants grown in medium. The results show that dwarfing in autotetraploid apple plants is most likely regulated by IAA and BR. The dwarf phenotype of autotetraploid apple plants could be due to accumulation of miR390 after genome doubling, leading to upregulation of apple trans-acting short-interfering RNA 3 (MdTAS3) expression, which in turn downregulates the expression of MdARF3. Overall, this leads to partial interruption of the IAA and BR signal transduction pathway. Our study provides important insights into the molecular mechanisms underlying dwarfism in autopolyploid apple plants.

引用

页数：14

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