ISOLATION AND CHARACTERIZATION OF 20 MICROSATELLITE LOCI FOR LAUREL SPECIES (LAURUS, LAURACEAE)

被引：12

作者：

Arroyo, Juan M. ^{[1
]}

Rigueiro, Cristina ^{[1
]}

Rodriguez, Rocio ^{[1
]}

Hampe, Arndt ^{[1
]}

Valido, Alfredo ^{[1
]}

Rodriguez-Sanchez, Francisco ^{[2
]}

Jordano, Pedro ^{[1
]}

机构：

[1] CSIC, Estac Biol Donana, Dept Integrat Ecol, E-41092 Seville, Spain

[2] Univ Seville, Dept Biol Vegetal & Ecol, E-41080 Seville, Spain

来源：

AMERICAN JOURNAL OF BOTANY | 2010年 / 97卷 / 05期

关键词：

genetic diversity; Laurus azorica; Laurus nobilis; polyploidy; SSR;

D O I：

10.3732/ajb.1000069

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n-enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively. Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.

引用

页码：E26 / E30

页数：5

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