Transcriptional analysis of porcine circovirus-like virus P1

被引：14

作者：

Wen, Li-bin ^{[1
]}

Wang, Feng-zhi ^{[1
]}

He, Kong-wang ^{[1
]}

Li, Bin ^{[1
]}

Wang, Xiao-min ^{[1
]}

Guo, Rong-li ^{[1
]}

Xie, Jian-ping ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Minist Agr, Natl Ctr Engn Res Vet Bioprod,Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

来源：

BMC VETERINARY RESEARCH | 2014年 / 10卷

基金：

中国国家自然科学基金;

关键词：

Porcine circovirus like virus P1; Transcriptional analysis; Northern blotting; RACE; COMPLETE GENOME SEQUENCE; IN-VITRO; TYPE-2; AGENT; IDENTIFICATION; PROTEIN; CELLS;

D O I：

10.1186/s12917-014-0287-3

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts. Results: The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids). Conclusions: We show a very complex viral transcription pattern in P1-infected cells.

引用

页数：8

共 23 条

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