Functional expression of pharaonis phoborhodopsin in Escherichia coli

被引：117

作者：

Shimono, K ^{[1
]}

Iwamoto, M ^{[1
]}

Sumi, M ^{[1
]}

Kamo, N ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Biophys Chem Lab, Sapporo, Hokkaido 060, Japan

来源：

FEBS LETTERS | 1997年 / 420卷 / 01期

关键词：

pharaonis phoborhodopsin (ppR); pharaonis sensory rhodopsin II (psRII); eubacterial expression; photocycle; flash-photolysis;

D O I：

10.1016/S0014-5793(97)01487-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial. Natronobacterium pharaonis, was functionally expressed in the heterologous system of Escherichia coli. Flash-photolysis on a millisecond time scale indicated that the photochemical properties of ppR expressed in E. coli were the same as those of the native ppR in N. pharaonis. Wt concluded that the integral membrane protein ppR is correctly folded in vivo in the eubacterial E, coli membrane. (C) 1997 Federation of European Biochemical Societies.

引用

页码：54 / 56

页数：3

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