Inhibition of benzoic acid on the polyaniline-polyphenol oxidase biosensor

被引：21

作者：

Li, Shengqi ^{[1
]}

Tan, Yongyan ^{[1
]}

Wang, Peng ^{[1
]}

Kan, Jinqing ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2010年 / 144卷 / 01期

基金：

美国国家科学基金会;

关键词：

Inhibition; Benzoic acid; Polyphenol oxidase; Biosensor; Polyaniline; SOLID-PHASE MICROEXTRACTION; MUSHROOM TYROSINASE; ENZYME; CHROMATOGRAPHY; PERFORMANCE; ELECTRODES;

D O I：

10.1016/j.snb.2009.08.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A polyaniline-polyphenol oxidase (PANI-PPO) biosensor for detecting benzoic acid is reported. The biosensor is based on the inhibition of benzoic acid on the biocatalytic activity of the polyphenol oxidase (PPO). The Michaelis-Menten constant (k(m)') and maximum response current(I-max) in both the absence and presence of benzoic acid are also evaluated. The kinetic analyses show that the inhibition of benzoic acid on the PPO activity is reversible and competitive with an inhibition constant of 28.7 mu mol dm(-3) A limit of detection of 0.3 mu mol dm(-3) benzoic acid is obtained. The I-0.5 value is estimated to be 35 mu mol dm(-3). The biosensor has good sensitivity and high stability. So it can be used as a new approach for the detecting benzoic acid. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：18 / 22

页数：5

共 34 条

[11] Further studies on the enzyme, tyrosinase [J].

Gregg, DC ;

Nelson, JM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1940, 62 :2500-2505

[12] Direct spectrophotometric assay of monooxygenase and oxidase activities of mushroom tyrosinase in the presence of synthetic and natural substrates [J].

Haghbeen, K ;

Tan, EW .

ANALYTICAL BIOCHEMISTRY, 2003, 312 (01) :23-32

[13] INHIBITION OF MUSHROOM TYROSINASE BY TROPOLONE [J].

KAHN, V ;

ANDRAWIS, A .

PHYTOCHEMISTRY, 1985, 24 (05) :905-908

[14] THE INHIBITION OF TYROSINASE [J].

KRUEGER, RC .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1955, 57 (01) :52-60

[15] SOME INHIBITORS OF MUSHROOM CATECHOLASE [J].

KUTTNER, R ;

WAGREICH, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1953, 43 (01) :80-87

[16]

Ludwig B.J., 1939, J Am. Chem. Soc, V61, P2601

[17] Design of a composite amperometric enzyme electrode for the control of the benzoic acid content in food [J].

Morales, MD ;

Morante, S ;

Escarpa, A ;

González, MC ;

Reviejo, AJ ;

Pingarrón, JM .

TALANTA, 2002, 57 (06) :1189-1198

[18] Preventing inhibition of tyrosinase with modified electrodes [J].

Narli, Isil ;

Kiralp, Senem ;

Toppare, Levent .

ANALYTICA CHIMICA ACTA, 2006, 572 (01) :25-31

[19]

*NAT STAND CHIN, 2003, GBT5009292003 NAT ST

[20] Determination of the antibacterial enrofloxacin by differential-pulse adsorptive stripping voltammetry [J].

Navalón, A ;

Blanc, R ;

Reyes, L ;

Navas, N ;

Vílchez, JL .

ANALYTICA CHIMICA ACTA, 2002, 454 (01) :83-91

← 1 2 3 4 →