Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report

被引：37
作者：
Zhang, Keshan [1 ]
Lu, Zhongxin [1 ]
Shang, Youjun [1 ]
Zheng, Haixue [1 ]
Jin, Ye [1 ]
He, Jijun [1 ]
Liu, Xiangtao [1 ]
机构：
[1] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Natl Foot & Mouth Dis Reference Lab, Key Lab Anim Virol,Minist Agr,Lanzhou Vet Res Ins, Lanzhou 730046, Gansu, Peoples R China
来源：
VIROLOGY JOURNAL | 2010年 / 7卷
关键词：
CONTAGIOUS PUSTULAR DERMATITIS; PARAPOXVIRUS; INFECTION; IDENTIFICATION; PREVALENCE; LAMBS; SHEEP; RISK;
D O I：
10.1186/1743-422X-7-78
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Background: Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants. Case presentation: In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene. Conclusion: The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.
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页数：5
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