Draft genomes of Cronobacter sakazakii strains isolated from dried spices bring unique insights into the diversity of plant-associated strains

被引：17

作者：

Jang, Hyein ^{[1
]}

Woo, Jungha ^{[1
]}

Lee, Youyoung ^{[1
]}

Negrete, Flavia ^{[1
]}

Finkelstein, Samantha ^{[1
]}

Chase, Hannah R. ^{[1
]}

Addy, Nicole ^{[1
]}

Ewing, Laura ^{[1
]}

Beaubrun, Junia Jean Gilles ^{[1
]}

Patel, Isha ^{[1
]}

Gangiredla, Jayanthi ^{[1
]}

Eshwar, Athmanya ^{[6
]}

Jaradat, Ziad W. ^{[2
]}

Seo, Kunho ^{[3
]}

Shabarinath, Srikumar ^{[4
,5
]}

Fanning, Seamus ^{[4
,5
]}

Stephan, Roger ^{[6
]}

Lehner, Angelika ^{[6
]}

Tall, Ben D. ^{[1
]}

Gopinath, Gopal R. ^{[1
]}

机构：

[1] US FDA, Ctr Food Safety & Appl Nutr, 8301 Muirkirk Rd, Laurel, MD 20708 USA

[2] Jordan Univ Sci & Technol, Dept Nutr & Food Technol, Irbid 22110, Jordan

[3] Konkuk Univ, Coll Vet Med, Ctr Hlth 1, Seoul 05029, South Korea

[4] Univ Coll, UCD Ctr Food Safety, Sch Publ Hlth Physiotherapy & Populat Sci, Dublin, Ireland

[5] WHO Collaborating Ctr Cronobacter, Dublin 4, Ireland

[6] Univ Zurich, Inst Food Safety & Hyg, Zurich, Switzerland

来源：

STANDARDS IN GENOMIC SCIENCES | 2018年 / 13卷

关键词：

Cronobacter sakazakii; WGS; Draft Genomes; Plant-origin; Dried Spices; POWDERED INFANT FORMULA; ENTEROBACTER-SAKAZAKII; SEQUENCE; PATHOGEN; DESICCATION; PHYLOGENIES; INFECTIONS; MECHANISMS; PERSISTENT; GENUS;

D O I：

10.1186/s40793-018-0339-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of C. sakazakii, which were obtained from dried spices from the USA, the Middle East, China, and the Republic of Korea. The average genome size of the C. sakazakii genomes was 4393kb, with an average of 4055 protein coding genes, and an average genome G+C content of 56.9%. The genomes contained genes related to carbohydrate transport and metabolism, amino acid transport and metabolism, and cell wall/membrane biogenesis. In addition, we identified genes encoding proteins involved in osmotic responses such as DnaJ, Aquaproin Z, ProQ, and TreF, as well as virulence-related and heat shock-related proteins.Interestingly, a metabolic island comprised of a variably-sized xylose utilization operon was found within the spice-associated C. sakazakii genomes, which supports the hypothesis that plants may serve as transmission vectors or alternative hosts for Cronobacter species. The presence of the genes identified in this study can support the remarkable phenotypic traits of C. sakazakii such as the organism's capabilities of adaptation and survival in response to adverse growth environmental conditions (e.g. osmotic and desiccative stresses). Accordingly, the genome analyses provided insights into many aspects of physiology and evolutionary history of this important foodborne pathogen.

引用

页数：16