Imaging protein behavior inside the living cell

被引：15
作者：
Day, RN [1 ]
机构：
[1] Univ Virginia Hlth Syst, Dept Med, Charlottesville, VA 22908 USA
[2] Univ Virginia Hlth Syst, Dept Cell Biol, Charlottesville, VA 22908 USA
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2005年 / 230卷 / 1-2期
关键词：
bioimaging; quantitative fluorescence microscopy; green fluorescent protein; nuclear structure; FRET; FLIM;
D O I：
10.1016/j.mce.2004.10.011
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The genetically encoded fluorescent proteins (FPs) have transformed studies in cell biology by allowing the behavior of proteins to be tracked within the natural environment of the living cell. Progressively more complex imaging methods are being used to measure the mobility, co-localization and interactions of proteins labeled with the FPs. This review provides an overview of recent developments in live-cell imaging techniques to analyze the subcellular distribution and interactions of proteins in living cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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页码：1 / 6
页数：6
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