Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis

被引：29
作者：
Liu, Hong-Tao [2 ,3 ]
He, Jun-Lin [2 ]
Li, Wen-Ming [2 ]
Yang, Zhu [2 ]
Wang, Ying-Xiong [2 ]
Bai, Xue-Fang
Yu, Chao [1 ,2 ]
Du, Yu-Guang
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Lab Nat Prod & Glyco Engn Res, Dalian 116023, Peoples R China
[2] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China
[3] Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2010年 / 80卷 / 04期
关键词：
Chitosan oligosaccharides; Hydrogen peroxide; Apoptosis; Human umbilical vein endothelial cell; OXIDATIVE STRESS; UP-REGULATION; CHITOOLIGOSACCHARIDES; SURVIVAL; ACTIVATION; MECHANISMS; INHIBITION; CALCIUM; BCL-2; DEATH;
D O I：
10.1016/j.carbpol.2010.01.025
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
This study aimed to investigate the protective effect of chitosan oligosaccharides (COS) on human umbilical vein endothelial cell (HUVEC) apoptosis induced by hydrogen peroxide (H2O2). We found that COS not only reversed the decrease of cell viability and proliferation activity, but ameliorated nuclear chromatin damage in H2O2-induced HUVECs. Additionally, COS contributed to the decrease of cytosolic Ca2+ level, increase of mitochondrial membrane potential, up-regulation of Bcl-2 mRNA, down-regulation of Bax mRNA, reduction of cleaved caspase-3 protein, inhibition of phosphorylated p38 mitogen-activated protein kinase (MAPK) and induction of phosphorylated Akt in HUVECs. Further, H2O2-induced caspase-3 activation could be suppressed by p38 MAPK inhibitor (SB203580) and up-regulated by phosphatidylinositol-3-kinase (PI3K) inhibitor (LY294002), indicating the involvement of p38 MAPK and PI3K/Akt signaling pathways. In conclusion, the results show that COS may effectively inhibit the H2O2-induced HUVEC apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1062 / 1071
页数：10
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