β-adrenergic receptor density and adenylate cyclase activity in lead-exposed rat brain after cessation of lead exposure

被引:8
作者
Chang, HR
Tsao, DA
Yu, HS
Ho, CK
机构
[1] I Shou Univ, Dept Biomed Engn, Kaohsiung, Taiwan
[2] Fooyin Univ Technol, Dept Med Technol, Kaohsiung, Taiwan
[3] Taiwan Natl Univ, Coll Med, Dept Dermatol, Taipei, Taiwan
[4] Kaohsiung Med Univ, Grad Inst Med, Res Ctr Occupat Dis, Kaohsiung, Taiwan
关键词
lead; beta-adrenoceptor; adenylate cyclase activity; brain;
D O I
10.1007/s00204-004-0605-9
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
To understanding the reversible or irreversible harm to the beta-adrenergic system in the brain of lead-exposed rats, this study sets up an animal model to estimate the change in the sympathetic nervous system of brain after lead exposure was withdrawn. We address the following topics in this study: ( a) the relationship between withdrawal time of lead exposure and brain beta-adrenergic receptor, blood lead level, and brain lead level in lead-exposed rats after lead exposure was stopped; and (b) the relationship between lead level and beta-adrenergic receptor and cyclic AMP (c-AMP) in brain. Wistar rats were chronically fed with 2% lead acetate and water for 2 months. Radioligand binding was assayed by a method that fulfilled strict criteria of beta-adrenergic receptor using the ligand [I-125] iodocyanopindolol. The levels of lead were determined by electrothermal atomic absorption spectrometry. The c-AMP level was determined by radioimmunoassay. The results showed a close relationship between decreasing lead levels and increasing numbers of brain beta-adrenergic receptors and brain adenylate cyclase activity after system of brain after lead exposure was withdrawn. We address the following topics in this study: ( a) the relationship between withdrawal time of lead exposure and brain beta-adrenergic receptor, blood lead level, and brain lead level in lead-exposed rats after lead exposure was stopped; and (b) the relationship between lead level and beta-adrenergic receptor and cyclic AMP (c-AMP) in brain. Wistar rats were chronically fed with 2% lead acetate and water for 2 months. Radioligand binding was assayed by a method that fulfilled strict criteria of beta-adrenergic receptor using the ligand [ 125 I] iodocyanopindolol. The levels of lead were determined by electrothermal atomic absorption spectrometry. The c-AMP level was determined by radioimmunoassay. The results showed a close relationship between decreasing lead levels and increasing numbers of brain beta-adrenergic receptors and brain adenylate cyclase activity after lead exposure was withdrawn. The effect of lead exposure on the beta-adrenergic system of the brain is a partly reversible condition.
引用
收藏
页码:1 / 6
页数:6
相关论文
共 34 条
[1]  
BAKER EL, 1985, BRIT J IND MED, V42, P507
[2]  
BAKER EL, 1984, BRIT J IND MED, V41, P352
[3]   LEAD NEPHROPATHY [J].
BENNETT, WM ;
KASSIRER, JP ;
PORTER, GA ;
PARKER, R ;
ELZINGA, L ;
KAUFFMAN, S .
KIDNEY INTERNATIONAL, 1985, 28 (02) :212-220
[4]  
BOOZE RM, 1989, J PHARMACOL EXP THER, V249, P911
[5]   BEHAVIORAL-EFFECTS OF MODERATE LEAD-EXPOSURE IN CHILDREN AND ANIMAL-MODELS .2. ANIMAL STUDIES [J].
BORNSCHEIN, R ;
PEARSON, D ;
REITER, L .
CRC CRITICAL REVIEWS IN TOXICOLOGY, 1980, 8 (02) :101-152
[6]   SIMPLE AND SENSITIVE SATURATION ASSAY METHOD FOR MEASUREMENT OF ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE [J].
BROWN, BL ;
ALBANO, JDM ;
EKINS, RP ;
SGHERZI, AM ;
TAMPION, W .
BIOCHEMICAL JOURNAL, 1971, 121 (03) :561-+
[7]   Health care costs [J].
Chant, ADB .
NATURE MEDICINE, 1996, 2 (01) :5-5
[8]  
CHISOLM JJ, 1975, JOHNS HOPKINS MED J, V137, P6
[10]  
DIBNER MD, 1979, J PHARMACOL EXP THER, V210, P433