First molecular detection of Babesia vogeli in dogs from Brazil

被引：88

作者：

Passos, LMF

Geiger, SM

Ribeiro, MFB

Pfister, K

Zahler-Rinder, M

机构：

[1] Univ Fed Minas Gerais, Escola Vet, Dept Vet Prevent Med, BR-30123970 Belo Horizonte, MG, Brazil

[2] Univ Munich, Inst Comparat Trop Med & Parasitol, D-80802 Munich, Germany

[3] Univ Fed Minas Gerais, ICB, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil

[4] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoal Dis, Obihiro, Hokkaido 0808555, Japan

来源：

VETERINARY PARASITOLOGY | 2005年 / 127卷 / 01期

关键词：

Babesia vogeli; Babesia canis; PCR; 18S rDNA; dog; Brazil;

D O I：

10.1016/j.vetpar.2004.07.028

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The present work describes the detection and first molecular characterization of Babesia vogeli in dogs, naturally infected in Brazil and even in South America. Microscopic examination of Giemsa-stained peripheral blood smears collected from dogs originating from four different locations in Brazil revealed the presence of large Babesia merozoites and trophozoites (>2.5 mum). DNA was extracted from infected blood samples and PCR amplifications of the 18S rDNA were carried out. As a reference, DNA from an isolate of B. vogeli originated from Egypt was used. PCR products were purified and sequenced. The DNA sequences demonstrated 100% identity among the Brazilian isolates. Comparisons with the 18S rDNA sequence of the B. vogeli isolate from Egypt and with other B. vogeli sequences from Spain, France, Japan, Australia and South Africa confirmed the affiliation of all Brazilian isolates to the species B. vogeli. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：81 / 85

页数：5

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