In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

被引：6

作者：

Luis Pina-Escutia, Jose ^{[1
]}

Miguel Vazquez-Garcia, Luis ^{[1
]}

Martin Arzate-Fernandez, Amaury ^{[1
]}

机构：

[1] Univ Autonoma Estado Mexico, Fac Ciencias Agr, Ctr Invest & Estudios Avanzados Fitomejoramiento, Toluca 50200, Estado Mexico, Mexico

来源：

ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2010年 / 13卷 / 01期

关键词：

genetic integrity; micropropagation; oceloxochitl; THUNB; VAR; BUKOSANENSE; SOMATIC EMBRYOGENESIS; PLANTS; STABILITY; ORGANOGENESIS; PROPAGATION; CULTURE; RAPD;

D O I：

10.2225/vol13-issue1-fulltext-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N-6 basal medium. The highest formation of shoots per responding explant was obtained on N-6 medium supplemented with 4.5 mu M 2,4-dichlorophenoxy acetic acid in combination with 2.2 mu M benzylaminopurine. Shoots rooted readily on N-6 basal medium supplemented with 1 g l(-1) activated charcoal and 2.6 mu M naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.

引用

页数：7

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