A novel and rapid approach to yeast differentiation using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

被引：7
|
作者：
Sherburn, RE [1 ]
Jenkins, RO [1 ]
机构：
[1] De Montfort Univ, Sch Mol Sci, Leicester LE1 9BH, Leics, England
来源：
SPECTROSCOPY-AN INTERNATIONAL JOURNAL | 2003年 / 17卷 / 01期
关键词：
D O I：
10.1155/2003/849530
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF-MS) was investigated as a method for the rapid identification of yeast cells. Following pretreatment of yeast samples with a cell wall digesting enzyme (lyticase), distinct and reproducible mass spectra over the m/z range 2,000 to 16,000 were obtained by MALDI-TOF-MS. Using an optimised procedure, characteristic mass spectra that distinguished between Candida spp. and between strains of Saccharomyces cerevisiae were produced. The approach offers the potential for rapid differentiation of yeasts in clinical diagnosis and in the fermentation industries.
引用
收藏
页码：31 / 38
页数：8
相关论文
共 50 条
  • [41] Identification of isoenzymes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Hardouin, J
    Hubert-Roux, M
    Delmas, AF
    Lange, C
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (05) : 725 - 732
  • [42] Rapid detection of silkworm microsporidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Kajiwara, Hideyuki
    Murakami, Ritsuko
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2019, 166
  • [43] Rapid quantitation of potato glycoalkaloids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Abell, DC
    Sporns, P
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (08) : 2292 - 2296
  • [44] Rapid characterization of polyalcohols by silylation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Adeuya, Anthony
    Price, Neil
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (24) : 3977 - 3981
  • [45] Identification of bacteria using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Kedney, Mollie G.
    Strunk, Kevin B.
    Giaquinto, Lisa M.
    Wagner, Jennifer A.
    Pollack, Sidney
    Patton, Walter A.
    BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, 2007, 35 (06) : 425 - 433
  • [46] Adventures with ribosomes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Hamburg, Daisy-Malloy
    Limbach, Patrick A.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 231
  • [47] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of mold and yeast cultures of Penicillium marneffei
    Lau, Susanna K. P.
    Lam, Clare S. K.
    Ngan, Antonio H. Y.
    Chow, Wang-Ngai
    Wu, Alan K. L.
    Tsang, Dominic N. C.
    Tse, Cindy W. S.
    Que, Tak-Lun
    Tang, Bone S. F.
    Woo, Patrick C. Y.
    BMC MICROBIOLOGY, 2016, 16
  • [48] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of mold and yeast cultures of Penicillium marneffei
    Susanna K. P. Lau
    Clare S. K. Lam
    Antonio H. Y. Ngan
    Wang-Ngai Chow
    Alan K. L. Wu
    Dominic N. C. Tsang
    Cindy W. S. Tse
    Tak-Lun Que
    Bone S. F. Tang
    Patrick C. Y. Woo
    BMC Microbiology, 16
  • [49] Quantification of hepcidin using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Bansal, Sukhvinder S.
    Halket, John M.
    Fusova, Jane
    Bomford, Adrian
    Simpson, Robert J.
    Vasavda, Nisha
    Thein, Swee Lay
    Hider, Robert C.
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2009, 23 (11) : 1531 - 1542
  • [50] A novel approach to identify plant parasitic nematodes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Perera, MR
    Vanstone, VA
    Jones, MGK
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (11) : 1454 - 1460
12345