Simultaneous determination of 8 kinds of organic acids in formula feed by capillary electrophoresis

被引：3

作者：

Ishikuro, E ^{[1
]}

Hibino, H ^{[1
]}

Soga, T ^{[1
]}

Yanai, H ^{[1
]}

Sawada, H ^{[1
]}

机构：

[1] Minist Agr Forestry & Fisheries, Sendai Fertilizer & Food Inspect Stn, Miyagino Ku, Sendai, Miyagi 9830842, Japan

来源：

JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN | 2000年 / 41卷 / 04期

关键词：

capillary electrophoresis; organic acid; formula feed; feed additive; ultrafiltration filter; collaborative study;

D O I：

10.3358/shokueishi.41.261

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An analytical method for organic acids (formic acid, fumaric acid, tartaric acid, malic acid, citric acid, acetic acid, lactic acid and propionic acid) in formula feeds by capillary electrophoresis (CE) was established. Organic acids were simultaneously extracted with water. The extract was filtered, diluted with 0.02 mol/L NaOH, and deproteinized by an ultrafiltration filter (30 kDa cut-off). The recovery test was conducted with 3 kinds of formula feed spiked with 8 kinds of organic acids at levels of 0.05 similar to 2.0%. The mean recovery values were in the range of 85.1 similar to 107.3%, and the relative standard deviation of repeatability (RSD) was within 13.6%. A collaborative study (3 laboratories) was conducted with formula feed spiked with 8 kinds of organic acids at levels of 0.1 similar to 1.0%. The mean recovery was 87.3 similar to 105.0%, and the relative standard deviation of reproducibility (RSDR) was within 16.3%.

引用

页码：261 / 267

页数：7

共 18 条

[1]

FUJIMURA K, 1988, BUNSEKI KAGAKU, V37, P549

[2]

HARUTA M, 1989, SHOKUHIN EISEI KENSA, P164

[3]

HIYAMA K, 1986, CHIKUSAN NO KENKYU, V40, P60

[4] EFFECTS OF A BUFFERED PROPIONIC-ACID IN DIETS ON THE PERFORMANCE OF BROILER-CHICKENS AND ON MICROFLORA OF THE INTESTINE AND CARCASS [J].