Advances in the detection of telomerase activity using isothermal amplification

被引：53

作者：

Zhang, Xiaojin ^{[1
]}

Lou, Xiaoding ^{[2
]}

Xia, Fan ^{[1
,2
]}

机构：

[1] China Univ Geosci, Fac Mat Sci & Chem, Wuhan 430074, Peoples R China

[2] Huazhong Univ Sci & Technol, Hubei Key Lab Bioinorgan Chem & Mat Med, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China

来源：

THERANOSTICS | 2017年 / 7卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Telomerase activity; isothermal amplification; electrochemical assays; optical assays; signal-transduction assays; PERSONAL GLUCOSE METERS; SINGLE-CELL LEVEL; TARGET RECYCLING AMPLIFICATION; FREE ULTRASENSITIVE DETECTION; HIGHLY SENSITIVE DETECTION; ENHANCED RAMAN-SCATTERING; GOLD NANOPARTICLE PROBE; BLADDER-CANCER PATIENTS; REAL-TIME DETECTION; INTRACELLULAR TELOMERASE;

D O I：

10.7150/thno.18930

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Telomerase plays a significantly important role in keeping the telomere length of a chromosome. Telomerase overexpresses in nearly all tumor cells, suggesting that telomerase could be not only a promising biomarker but also a potential therapeutic target for cancers. Therefore, numerous efforts focusing on the detection of telomerase activity have been reported from polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP) assays to PCR-free assays such as isothermal amplification in recent decade. In this review, we highlight the strategies for the detection of telomerase activity using isothermal amplification and discuss some of the challenges in designing future telomerase assays as well.

引用

页码：1847 / 1862

页数：16

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