Role of nerve growth factor in cutaneous wound healing: Accelerating effects in normal and healing-impaired diabetic mice

被引:197
作者
Matsuda, H
Koyama, H
Sato, H
Sawada, J
Itakura, A
Tanaka, A
Matsumoto, M
Konno, K
Ushio, H
Matsuda, K
机构
[1] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Clin, Tokyo 183, Japan
[2] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Internal Med, Tokyo 183, Japan
关键词
D O I
10.1084/jem.187.3.297
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Four full-thickness skin wounds made in normal mice led to the significant increase in levels of nerve growth factor (NGF) in sera and in wounded skin tissues. Since sialoadenectomy before the wounds inhibited the nse in serum levels of NGF, the NGF may be released from the salivary gland into the blood stream after the wounds. In contrast, the fact that messenger RNA and protein of NGF were detected in newly formed epithelial cells at the edge of the wound and fibroblasts consistent with the granulation tissue produced in the wound space, suggests that NGF was also produced at the wounded skin site. Topical application of NGF into the wounds accelerated the rate of wound healing in normal mice and in healing-impaired diabetic KK/Ta mice. This clinical effect of NGF was evaluated by histological examination; the increases in the degree of reepithelialization, the thickness of the granulation tissue, and the density of extracellular matrix were observed. NGF also increased the breaking strength of healing linear wounds in normal and diabetic mice. These findings suggested that NGF immediately and constitutively released in response to cutaneous injury may contribute to wound healing through broader biological activities, and NGF improved the diabetic impaired response of wound healing.
引用
收藏
页码:297 / 306
页数:10
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