Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence

被引：112

作者：

Fradkov, AF

Chen, Y

Ding, L

Barsova, EV

Matz, MV

Lukyanov, SA ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

[2] Clontech Labs Inc, Palo Alto, CA USA

来源：

FEBS LETTERS | 2000年 / 479卷 / 03期

关键词：

green fluorescent protein; red fluorescent protein; random mutagenesis; drFP593; drFP583;

D O I：

10.1016/S0014-5793(00)01895-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm, Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：127 / 130

页数：4

共 12 条

[1] FLUORESCENCE EFFECTS FROM CORALS IRRADIATED WITH ULTRA-VIOLET RAYS [J].