In vitro flowering of Withania somnifera Dunal. -: An important antitumor medicinal plant

被引：36

作者：

Saritha, K. V. ^{[1
]}

Naidu, C. V. ^{[1
]}

机构：

[1] Sri Venkateswara Univ, Dept Biotechnol, Plant Biotechnol Div, Tirupati 517502, Andhra Pradesh, India

来源：

PLANT SCIENCE | 2007年 / 172卷 / 04期

关键词：

in vitro flowering; in vitro fruiting; seed set; Withania somnifera;

D O I：

10.1016/j.plantsci.2006.12.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro flowering, in vitro fruiting and effective micropropagation protocol were studied in Withania somnifera, an antitumor medicinal plant using axillary bud explants. The Murashige and Skoog's medium (MS) supplemented with e-benzyl adenine (BA) 2.0 mg l(-1) and a-naphthalene acetic acid 0.1 mg l(-1) was found optimum for production of multiple shoots. The regenerated plantlets were found to form tiny green floral buds after 4-6 weeks of culture in MS medium supplemented with Kinetin (0.5-4.0 mg l(-1)) and indole-3-acetic acid (0.1 mg l(-1)). In vitro fruiting was observed in the presence of Kn (2.0 mg l(-1)) and IAA (0.1 mg l(-1)). This paper describes in vitro flowering system to overcome problems associated with flower growth and development as well as fruit and seed production in vitro. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：847 / 851

页数：5

共 29 条

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