Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm

被引：21
作者：
Arslan, Halil Ozancan [1 ]
Herrera, Carolina [1 ]
Malama, Eleni [1 ]
Siuda, Mathias [1 ]
Leiding, Claus [2 ]
Bollwein, Heinrich [1 ]
机构：
[1] Univ Zurich, Vetsuisse Fac, Clin Reprod Med, Zurich, Switzerland
[2] Besamungsverein Neustadt Ad Aisch, Neustadt, Germany
来源：
CRYOBIOLOGY | 2019年 / 91卷
关键词：
Bull sperm; Cryopreservation; Catalase; Sperm quality; In vitro fertilization; OXIDATIVE STRESS; SEMEN CRYOPRESERVATION; BOVINE SPERMATOZOA; DNA-DAMAGE; ANTIOXIDANT; MOTILITY; RAM; VIABILITY; INTEGRITY; MEMBRANE;
D O I：
10.1016/j.cryobiol.2019.10.200
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to investigate the effects of different concentrations of catalase in a TRIS-egg yolk extender on sperm quality and embryonic development after in vitro fertilization of frozen-thawed bull sperm. For this purpose, from each of 7 bulls 2 ejaculates were collected and diluted with a TRIS-egg yolk extender containing 0, 5, 10, 15 or 20 IU catalase/mL. Sperm quality was evaluated 0, 3, 6, 12 and 24 h after thawing by using computer assisted analysis of motility and by flow cytometric assays. Embryonic development was determined after in vitro fertilization of bovine oocytes. Semen diluted with TRIS-egg yolk extender containing different concentrations of catalase showed more motile sperm, more sperm with intact plasma membranes, acrosomes and DNA, a high mitochondrial membrane potential, a high esterase activity, a low calcium level, a lower amount of synthesis of reactive oxygen species and lower degree of lipid peroxidation of sperm compared to semen frozen without catalase (P < 0.05), but not before 3 h after thawing. There was a dose-response relationship with the most prominent effect of 20 IU catalase/mL. However, the improvement of sperm quality had no effect (P > 0.05) on embryonic development after in vitro fertilization with 20 IU catalase/mL. In conclusion, the addition of catalase to the sperm extender improved sperm quality with no obvious effect on in vitro fertility.
引用
收藏
页码：40 / 52
页数：13
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