Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

被引：12

作者：

Mangum, Lauren H. ^{[1
]}

Natesan, Shanmugasundaram ^{[1
]}

Stone, Randolph, II ^{[1
]}

Wrice, Nicole L. ^{[1
]}

Larson, David A. ^{[1
]}

Florell, Kyle F. ^{[1
]}

Christy, Barbara A. ^{[2
]}

Herzig, Maryanne C. ^{[2
]}

Cap, Andrew P. ^{[2
]}

Christy, Robert J. ^{[1
]}

机构：

[1] US Army, Combat Trauma & Burn Injury Res, Inst Surg Res, San Antonio Mil Med Ctr,JBSA Ft Sam Houston, San Antonio, TX 78234 USA

[2] US Army, Coagulat & Blood Res, Inst Surg Res, San Antonio Mil Med Ctr,JBSA Ft Sam Houston, San Antonio, TX USA

来源：

STEM CELLS INTERNATIONAL | 2017年 / 2017卷

关键词：

STROMAL-VASCULAR FRACTION; FETAL BOVINE SERUM; HUMAN PLATELET LYSATE; GENE-EXPRESSION; DIFFERENTIATION; PROLIFERATION; CULTURE; OPTIMIZATION; CAPACITY; THERAPY;

D O I：

10.1155/2017/7108458

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95-99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p < 0 05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes.

引用

页数：15

共 68 条

[1] Mesenchymal stem cells expanded in human platelet lysate display a decreased inhibitory capacity on T- and NK-cell proliferation and function [J].