Detection of chromosomal gains and losses in comparative genomic hybridization analysis based on standard reference intervals

被引：0

作者：

Kirchhoff, M

Gerdes, T

Rose, H

Maahr, J

Ottesen, AM

Lundsteen, C

机构：

[1] Univ Copenhagen, Chromosome Lab 4051,Rigshosp, Dept Clin Genet, Juliane Marie Ctr, DK-2100 Copenhagen O, Denmark

[2] Univ Copenhagen, Rigshosp, Juliane Marie Ctr, Dept Growth & Reprod, DK-2100 Copenhagen, Denmark

来源：

CYTOMETRY | 1998年 / 31卷 / 03期

关键词：

comparative genomic hybridization; CGH; quantitative image analysis; detection criteria; t-statistic;

D O I：

10.1002/(SICI)1097-0320(19980301)31:3<163::AID-CYTO3>3.3.CO;2-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Criteria for detection of chromosome aberrations by Comparative Genomic Hybridization (CGH) are not standardized and improvement of this part of the analysis is of paramount importance to the applicability of the technique. The aim of this work was to suggest CGH detection criteria that increase the specificity and sensitivity and at the same time include chromosome regions previously excluded from CGH analysis. We analyzed 33 hybridizations with normal DNA and modified our CGH software in order to use a selection of these normal analyses as a model for interpretation of analyses of unknown samples. This approach was successfully tested on 14 samples with known aberrations. (C) 1998 Wiley-Liss, Inc.

引用

页码：163 / 173

页数：11

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