Butylated hydroxyanisole alters rat 5α-reductase and 3α-hydroxysteroid dehydrogenase: Implications for influences of neurosteroidogenesis

被引：7
作者：
Guo, Jingjing [1 ]
Li, Lili [2 ,3 ]
Zhou, Songyi [2 ,3 ]
Su, Ying [2 ,3 ]
Li, Xiaoheng [2 ,3 ]
Sun, Jianliang [4 ]
Ge, Ren-Shan [2 ,3 ]
机构：
[1] Capital Med Univ, Beijing Chaoyang Hosp, Dept Anesthesiol, Beijing 100020, Peoples R China
[2] Wenzhou Med Univ, Dept Anesthesiol, Affiliated Hosp 2, Wenzhou 325027, Zhejiang, Peoples R China
[3] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China
[4] Nanjing Med Univ, Hangzhou Hosp, Dept Anesthesia, Hangzhou Peoples Hosp 1, Hangzhou 310006, Zhejiang, Peoples R China
来源：
NEUROSCIENCE LETTERS | 2017年 / 653卷
关键词：
Butylated hydroxyanisole; Neurosteroid; 5; alpha-reductase; 1; 3 alpha-hydroxysteroid dehydrogenase; Retinol dehydrogenase 2; KETO REDUCTASE SUPERFAMILY; DIHYDRODIOL DEHYDROGENASE; LEYDIG-CELLS; MOUSE MODEL; BRAIN; TESTOSTERONE; ALLOPREGNANOLONE; EXPRESSION; STRESS; IDENTIFICATION;
D O I：
10.1016/j.neulet.2017.05.034
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Butylated hydroxyanisole is a synthetic antioxidant. It may affect the function of the nerve system. The objective of the present study is to investigate the direct effects of butylated hydroxyanisole on rat brain neurosteroidogenic 5 alpha-reductase 1 (SRD5A1),3 alpha-hydroxysteroid dehydrogenase (AKR1C14), and retinol dehydrogenase 2 (RDH2). Rat SRD5A1, AKR1C14, and RDH2 were cloned and expressed in COS1 cells, and the effects of butylated hydroxyanisole on these enzyme activities were measured. Butylated hydroxyanisole inhibited SRD5A1, AKR1C14, and RDH2 with IC50 values of 4.731 +/- 0.079 mu M, 5.753 +/- 0.073 mu M, and over 100 mu M, respectively. Butylated hydroxyanisole is a competitive inhibitor for both SRD5A1
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页码：132 / 138
页数：7
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