Genes encoding tetracycline resistance in a full-scale municipal wastewater treatment plant investigated during one year

被引：43
作者：
Boerjesson, Stefan [1 ,2 ]
Mattsson, Ann
Lindgren, Per-Eric [1 ,2 ]
机构：
[1] Linkoping Univ, Dept Clin & Expt Med, Div Med Microbiol, SE-58185 Linkoping, Sweden
[2] Cty Hosp Ryhov, Dept Microbiol, SE-55185 Jonkoping, Sweden
来源：
JOURNAL OF WATER AND HEALTH | 2010年 / 8卷 / 02期
基金：
瑞典研究理事会; 英国医学研究理事会;
关键词：
LUX (TM) real-time polymerase chain reaction (PCR); tetA; tetB; tetracycline; wastewater treatment plant; ACTIVATED-SLUDGE; TREATMENT FACILITIES; BACTERIA; SEWAGE; ANTIBIOTICS; COMMUNITY; PLASMIDS; REMOVAL; UPDATE; TET;
D O I：
10.2166/wh.2009.159
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Tetracycline-resistant bacteria and genes encoding tetracycline resistance are common in anthropogenic environments. We studied how wastewater treatment affects the prevalence and concentration of two genes, tetA and tetB, that encode resistance to tetracycline. Using real-time polymerase chain reaction (PCR) we analysed wastewater samples collected monthly for one year at eight key-sites in a full-scale municipal wastewater treatment plant (WWTP). We detected tetA and tetB at each sampling site and the concentration of both genes, expressed per wastewater volume or per total-DNA, decreased over the treatment process. The reduction of tetA and tetB was partly the result of the sedimentation process. The ratio of tetA and tetB, respectively, to total DNA was lower in or after the biological processes. Taken together our data show that tetracycline resistance genes occur throughout the WWTP, and that the concentrations are reduced under conventional operational strategies.
引用
收藏
页码：247 / 256
页数：10
相关论文
共 20 条
[1]   Tetracycline resistance genes in activated sludge wastewater treatment plants [J].
Auerbach, Eric A. ;
Seyfried, Erin E. ;
McMahon, Katherine D. .
WATER RESEARCH, 2007, 41 (05) :1143-1151
[2]   Quantification of genes encoding resistance to aminoglycosides, β-lactams and tetracyclines in wastewater environments by real-time PCR [J].
Borjesson, Stefan ;
Dienues, Olaf ;
Jarnheimer, Per-Ake ;
Olsen, Bjorn ;
Matussek, Andreas ;
Lindgren, Per-Eric .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH, 2009, 19 (03) :219-230
[3]   A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant [J].
Borjesson, Stefan ;
Melin, Sara ;
Matussek, Andreas ;
Lindgren, Per-Eric .
WATER RESEARCH, 2009, 43 (04) :925-932
[4]   Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida [J].
Casas, C ;
Anderson, EC ;
Ojo, KK ;
Keith, I ;
Whelan, D ;
Rainnie, D ;
Roberts, MC .
FEMS MICROBIOLOGY LETTERS, 2005, 242 (01) :59-63
[5]   Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance [J].
Chopra, I ;
Roberts, M .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (02) :232-+
[6]   Fecal coliform removal in wastewater treatment plants studied by plate counts and enzymatic methods [J].
George, I ;
Crop, P ;
Servais, P .
WATER RESEARCH, 2002, 36 (10) :2607-2617
[7]  
Guillaume G, 2000, FEMS MICROBIOL ECOL, V32, P77, DOI 10.1016/S0168-6496(00)00016-7
[8]   Occurrence of antibiotics in wastewater treatment facilities in Wisconsin, USA [J].
Karthikeyan, KG ;
Meyer, MT .
SCIENCE OF THE TOTAL ENVIRONMENT, 2006, 361 (1-3) :196-207
[9]   Fate of tetracycline resistant bacteria as a function of activated sludge process organic loading and growth rate [J].
Kim, S. ;
Jensen, J. N. ;
Aga, D. S. ;
Weber, A. S. .
WATER SCIENCE AND TECHNOLOGY, 2007, 55 (1-2) :291-297
[10]   Tetracycline as a selector for resistant bacteria in activated sludge [J].
Kim, Sungpyo ;
Jensen, James N. ;
Aga, Diana S. ;
Weber, A. Scott .
CHEMOSPHERE, 2007, 66 (09) :1643-1651
12