Site-specific modification of enzymatically synthesized RNA: Transcription initiation and Diels-Alder reaction

被引：58
|
作者：
Seelig, B [1 ]
Jaschke, A [1 ]
机构：
[1] FREE UNIV BERLIN,INST BIOCHEM,D-14195 BERLIN,GERMANY
来源：
TETRAHEDRON LETTERS | 1997年 / 38卷 / 44期
关键词：
D O I：
10.1016/S0040-4039(97)10151-4
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Initiator nucleotides consisting of an anthracene ring system coupled to the 5'-phosphate of guanosine monophosphate via a polyethylene glycol (PEG) linker were chemically synthesized. When added to transcription reactions, these initiators were site-specifically incorporated into RNA transcripts yielding RNA conjugates with a fluorophore selectively attached to the 5'-end via a flexible linker. Besides sensitive fluorescence detection, the anthracene allowed the convenient modification with maleimides by Diels-Alder reaction. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：7729 / 7732
页数：4
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