Identification and quantification of histidine-rich glycoprotein (HRG) in the blood plasma of six marine bivalves

被引：20

作者：

Abebe, Adal T.

Devoid, Samantha J.

Sugumaran, Manickam

Etter, Ron

Robinson, William E.

机构：

[1] Univ Massachusetts, EEOS, Boston, MA 02125 USA

[2] Univ Massachusetts, Dept Biol, Boston, MA 02125 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2007年 / 147卷 / 01期

关键词：

bivalve; blood plasma; cadmium; ELISA; HRG; IMAC; metal carrier; transport protein;

D O I：

10.1016/j.cbpb.2006.12.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histidine-rich Glycoprotein (HRG) is a metal-binding protein described from the blood plasma of a pteriomorph bivalve, the marine mussel Mytilus edulis L. We demonstrate here, using Cd-Immobilized Metal Affinity Chromatography (IMAC), SDS-PAGE, Western Blotting, and ELISA, that HRG is present in three additional pteriomorphs and two heterodont bivalves. ELISA indicates that HRG is the predominant blood plasma protein in all six species (41 to 61% of total plasma proteins by weight). Thus, HRG appears to be a widespread metal-binding protein in the plasma of bivalves. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：74 / 81

页数：8

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