Production of isotope-labeled proteins in insect cells for NMR

被引：52

作者：

Franke, Bastian ^{[1
]}

Opitz, Christian ^{[1
]}

Isogai, Shin ^{[1
]}

Grahl, Anne ^{[1
]}

Delgado, Leonildo ^{[1
]}

Gossert, Alvar D. ^{[2
]}

Grzesiek, Stephan ^{[1
]}

机构：

[1] Univ Basel, Focal Area Struct Biol & Biophys, Biozentrum, CH-4056 Basel, Switzerland

[2] Swiss Fed Inst Technol, Dept Biol, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF BIOMOLECULAR NMR | 2018年 / 71卷 / 03期

基金：

瑞士国家科学基金会;

关键词：

Baculovirus; Yeast extract; Algal extract; Deuteration; GPCR; Abelson kinase; BETA(2)-ADRENERGIC RECEPTOR; ESCHERICHIA-COLI; BACULOVIRUS; ACID; EXPRESSION; COMPLEXES; GENERATION; DYNAMICS; PROTOCOL; N-15;

D O I：

10.1007/s10858-018-0172-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.

引用

页码：173 / 184

页数：12

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