Chronic Surgical Site Infection Due to Suture-Associated Polymicrobial Biofilm

被引：90

作者：

Kathju, Sandeep ^{[1
,2
]}

Nistico, Laura ^{[1
]}

Hall-Stoodley, Luanne ^{[1
,2
]}

Post, J. Christopher ^{[1
,2
]}

Ehrlich, Garth D. ^{[1
,2
]}

Stoodley, Paul ^{[1
,2
]}

机构：

[1] Allegheny Gen Hosp, Allegheny Singer Res Inst, Ctr Genom Sci, Pittsburgh, PA 15212 USA

[2] Drexel Univ, Coll Med, Dept Microbiol & Immunol, Pittsburgh, PA USA

来源：

SURGICAL INFECTIONS | 2009年 / 10卷 / 05期

关键词：

BACTERIAL BIOFILMS;

D O I：

10.1089/sur.2008.062

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Surgical site infection (SSI) is a common surgical complication; culture-negative SSI presents a particular problem in management. Methods: Examination of explanted foreign bodies (sutures) using confocal laser scanning microscopy (CLSM) and fluorescent in situ hybridization (FISH) after surgical exploration of a chronic culture-negative SSI. Results: Confocal microscopy (CM) demonstrated bacilli and cocci attached to the surface of the explanted sutures in a mixed biofilm. Florescent in situ hybridization confirmed that Staphylococci were components of the mixed biofilm. Removal of the foreign bodies (sutures) resolved the chronic infection. Conclusion: Chronic SSI can arise from underlying bacterial biofilms, which can invest implanted foreign bodies and associated soft tissue surfaces.

引用

页码：457 / 461

页数：5

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