Medium improvement for β-fructofuranosidase production by Aspergillus japonicus

被引：16
作者：
Chen, WC [1 ]
机构：
[1] Tatung Inst Technol, Dept Bioengn, Taipei 10451, Taiwan
来源：
PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 03期
关键词：
fructo-oligosaccharides; beta-fructofuranosidase; Aspergillus japonicus; experimental design;
D O I：
10.1016/S0032-9592(97)00066-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The medium composition for the production of intracellular beta-fructofuranosidase (FFase, EC 3.2.1.26) by Aspergillus japonicus TIT-90076 was improved by using statistical experimental designs and the method of steepest ascent in shake batch cultures. Sucrose and yeast extract were the key nutritional factors affecting enzyme production. Improved media containing 24.05% sucrose and 2.75% yeast extract increased enzyme production by 180%. (C) 1998 Elsevier Science Ltd.
引用
收藏
页码：267 / 271
页数：5
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