Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium

被引：118

作者：

Olmer, Ruth ^{[1
,8
]}

Haase, Alexandra ^{[1
,8
]}

Merkert, Sylvia ^{[1
,8
]}

Cui, Wei ^{[2
]}

Palecek, Jiri ^{[3
,8
]}

Ran, Chen ^{[4
,8
]}

Kirschning, Andreas ^{[3
,8
]}

Scheper, Thomas ^{[4
,8
]}

Glage, Silke ^{[5
,8
]}

Miller, Konstantin ^{[6
]}

Curnow, Eliza C. ^{[7
]}

Hayes, Eric S. ^{[7
]}

Martin, Ulrich ^{[1
,8
]}

机构：

[1] Hannover Med Sch, Leibniz Res Labs Biotechnol & Artificial Organs L, Dept Cardiac Thorac Transplantat & Vasc Surg, D-30625 Hannover, Germany

[2] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Inst Reprod & Dev Biol, Fac Med, London W12 ONN, England

[3] Leibniz Univ Hannover, Inst Organ Chem, Hannover, Germany

[4] Leibniz Univ Hannover, Inst Tech Chem, Hannover, Germany

[5] Hannover Med Sch, Inst Lab Anim Sci, D-30625 Hannover, Germany

[6] Hannover Med Sch, Dept Human Genet, D-30625 Hannover, Germany

[7] Washington Natl Primate Res Ctr, Seattle, WA USA

[8] Hannover Med Sch, REBIRTH Cluster Excellence, D-30625 Hannover, Germany

来源：

STEM CELL RESEARCH | 2010年 / 5卷 / 01期

关键词：

EMBRYONIC STEM-CELLS; GREEN FLUORESCENT PROTEIN; FEEDER-FREE; DIFFERENTIATION; PLURIPOTENCY; MAINTENANCE; EXPRESSION; GENERATION; MATRIX;

D O I：

10.1016/j.scr.2010.03.005

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Therapeutic application of stem cell derivatives requires large quantities of cells produced in defined media that cannot be produced via conventional adherent culture. We have applied human induced pluripotent stem (hiPS) cells expressing eGFP under control of the OCT4 promoter to establish the expansion of undifferentiated human embryonic stem (hES) and hiPS cells in suspension culture. A defined culture medium has been identified that results in up to six-fold increase in cell numbers within four days. Our culture system is based on initial single cell dissociation which is critical for standardized process inoculation. HES / hiPS cells were expanded for up to 17 passages. The cells maintained a stable karyotype, their expression of pluripotency markers and their potential to differentiate into derivatives of all three germ layers. The ability to expand HES / hiPS cells in a scalable suspension culture represents a critical step towards standardized production in stirred bioreactors. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：51 / 64

页数：14

共 27 条

[1] Customized molecular phenotyping by quantitative gene expression and pattern recognition analysis [J].