Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon

被引：49

作者：

Espadas, Javier ^{[1
,2
]}

Pendin, Diana ^{[3
,10
]}

Bocanegra, Rebeca ^{[4
]}

Escalada, Artur ^{[1
,2
]}

Misticoni, Giulia ^{[3
]}

Trevisan, Tatiana ^{[3
]}

Velasco del Olmo, Ariana ^{[1
,2
]}

Montagna, Aldo ^{[3
]}

Bova, Sergio ^{[5
]}

Ibarra, Borja ^{[4
,6
]}

Kuzmin, Peter, I ^{[7
]}

Bashkirov, Pavel, V ^{[8
]}

Shnyrova, Anna, V ^{[1
,2
]}

Frolov, Vadim A. ^{[1
,2
,9
]}

Daga, Andrea ^{[3
]}

机构：

[1] Univ Basque Country, Biofis Inst, CSIC, UPV EHU, Leioa 48940, Spain

[2] Univ Basque Country, Dept Biochem & Mol, Biol, Leioa 48940, Spain

[3] IRCCS E Medea, Lab Mol Biol, Sci Inst, Bosisio Parini, Lecco, Italy

[4] IMDEA Nanociencia, C Faraday 9,Ciudad Univ Cantoblanco, Madrid 28049, Spain

[5] Univ Padua, Dept Pharmaceut & Pharmacol Sci, Padua, Italy

[6] CSIC, Unidad Asociada Ctr Nacl Biotecnol, Nanobiotecnol IMDEA Nanociencia, Madrid 28049, Spain

[7] Russian Acad Sci, AN Frumkin Inst Phys Chem & Electrochem, Moscow 119071, Russia

[8] Fed Res & Clin Ctr Phys Chem Med, Moscow 119435, Russia

[9] Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain

[10] Italian Natl Res Council CNR, Neurosci Inst, Padua, Italy

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

俄罗斯科学基金会;

关键词：

PROTEINS; RECONSTITUTION; NANOSCALE; NETWORK; FUSION; CELLS; IMAGE;

D O I：

10.1038/s41467-019-13327-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The endoplasmic reticulum (ER) is a continuous cell-wide membrane network. Network formation has been associated with proteins producing membrane curvature and fusion, such as reticulons and atlastin. Regulated network fragmentation, occurring in different physiological contexts, is less understood. Here we find that the ER has an embedded fragmentation mechanism based upon the ability of reticulon to produce fission of elongating network branches. In Drosophila, Rtnl1-facilitated fission is counterbalanced by atlastin-driven fusion, with the prevalence of Rtnl1 leading to ER fragmentation. Ectopic expression of Drosophila reticulon in COS-7 cells reveals individual fission events in dynamic ER tubules. Consistently, in vitro analyses show that reticulon produces velocity-dependent constriction of lipid nanotubes leading to stochastic fission via a hemifission mechanism. Fission occurs at elongation rates and pulling force ranges intrinsic to the ER, thus suggesting a principle whereby the dynamic balance between fusion and fission controlling organelle morphology depends on membrane motility.

引用

页数：11

共 61 条

[1]

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