Increased Viability of Odontoblast-Like Cells Subjected to Low-Level Laser Irradiation

被引:35
作者
Oliveira, C. F. [1 ]
Basso, F. G. [2 ]
Lins, E. C. [3 ]
Kurachi, C. [3 ]
Hebling, J. [1 ]
Bagnato, V. S. [3 ]
de Souza Costa, C. A. [1 ]
机构
[1] UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
[2] UNICAMP Univ Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP, Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
ER-YAG LASER; DENTIN HYPERSENSITIVITY; PROMOTES PROLIFERATION; THERAPY; FIBROBLASTS; ATTACHMENT; LIGHT; DIFFERENTIATION; MECHANISMS; EXPRESSION;
D O I
10.1134/S1054660X10130153
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Studies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at 20000 cells/cm(2) were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO2 at 37 degrees C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm(2) + 5% FBS; G2: 1.5 J/cm(2) + 10% FBS; G3: 5 J/cm(2) + 5% FBS; G4: 5 J/cm(2) + 10% FBS; G5: 19 J/cm(2) + 5% FBS; G6: 19 J/cm(2) + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly (Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed to a laser dose of 1.5 J/cm(2). These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype.
引用
收藏
页码:1659 / 1666
页数:8
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