Transient gene expression of β-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment

被引：10

作者：

Bespalhok, JC ^{[1
]}

Kobayashi, AK ^{[1
]}

Pereira, LFP ^{[1
]}

Galvao, RM ^{[1
]}

Vieira, LGE ^{[1
]}

机构：

[1] Inst Agron Parana, Lab Biotecnol Vegetal, Area Melhoramento & Genet, BR-86001970 Londrina, PR, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2003年 / 46卷 / 01期

关键词：

transformation; uidA expression; biolistics; transgenic citrus;

D O I：

10.1590/S1516-89132003000100001

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions,for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h of er bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis).

引用

页码：1 / 6

页数：6

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