In vitro study of the proliferation and growth of human bone marrow cells on apatite-wollastonite-2M glass ceramics

被引：36

作者：

Magallanes-Perdomo, M. ^{[1
]}

De Aza, A. H. ^{[1
]}

Mateus, A. Y. ^{[2
,3
]}

Teixeira, S. ^{[2
,3
]}

Monteiro, F. J. ^{[2
,3
]}

De Aza, S. ^{[1
]}

Pena, P. ^{[1
]}

机构：

[1] CSIC, Inst Ceram & Vidrio, Madrid 28049, Spain

[2] INEB Inst Engn Biomed, Lab Biomat, P-4150180 Oporto, Portugal

[3] FEUP, Dept Met & Mat Engn, P-4200465 Oporto, Portugal

来源：

ACTA BIOMATERIALIA | 2010年 / 6卷 / 06期

关键词：

Glass ceramics; Cell culture; Apatite; Wollastonite; Cell proliferation; WOLLASTONITE-TRICALCIUM PHOSPHATE; MESENCHYMAL STEM-CELLS; OSTEOBLAST-LIKE CELLS; TITANIUM SURFACE-ROUGHNESS; MEDICAL APPLICATIONS; DIFFERENTIATION; BEHAVIOR; BIOCERAMICS; APATITE; SYSTEM;

D O I：

10.1016/j.actbio.2009.12.027

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This study concerns the preparation and in vitro characterization of an apatite-wollastonite-2M bioactive glass ceramic which is intended to be used for the regeneration of hard tissue (i.e. in dental and cranio-maxillofacial surgery). This bioglass ceramic has been obtained by appropriate thermal treatment through the devitrification (crystallization) of a glass with a stoichiometric eutectic composition within the Ca(3)(PO(4))(2)-CaSiO(3) binary system. Crack-free specimens of the bioglass ceramic were immersed in human bone marrow cell cultures for 3, 7, 14 and 21 days, in order to study biocompatibility. Cell morphology, proliferation and colonization were assessed by scanning electron microscopy and confocal laser scanning microscopy. A total protein content assay was used to evaluate the viability and proliferation of cultured bone marrow cells. The results showed that the cells were able to adhere and proliferate on the designed material due to the essentiality of silicon and calcium as accessory factors for cell activity stimulation. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：2254 / 2263

页数：10

共 45 条

[1] Bioceramics: Past, present and for the future
Best, S. M.
Porter, A. E.
Thian, E. S.
Huang, J.
[J]. JOURNAL OF THE EUROPEAN CERAMIC SOCIETY, 2008, 28 (07) : 1319 - 1327
[2] Bowers K T, 1992, Int J Oral Maxillofac Implants, V7, P302
[3] Boyan BD, 1998, J BIOMED MATER RES, V39, P77, DOI 10.1002/(SICI)1097-4636(199801)39:1<77::AID-JBM10>3.0.CO
[4] 2-L
[5] BROWN L, 2004, 7 WORLD BIOM C SYDN
[6] SILICON REQUIREMENT FOR NORMAL SKULL FORMATION IN CHICKS
CARLISLE, EM
[J]. JOURNAL OF NUTRITION, 1980, 110 (02) : 352 - 359
[7] BIOCHEMICAL AND MORPHOLOGICAL-CHANGES ASSOCIATED WITH LONG-BONE ABNORMALITIES IN SILICON DEFICIENCY
CARLISLE, EM
[J]. JOURNAL OF NUTRITION, 1980, 110 (05) : 1046 - 1056
[8] Ceramics for medical applications: A picture for the next 20 years
Chevalier, J.
Gremillard, L.
[J]. JOURNAL OF THE EUROPEAN CERAMIC SOCIETY, 2009, 29 (07) : 1245 - 1255
[9] In situ bone-like apatite formation from a Bioeutectic® ceramic in SBF dynamic flow
De Aza, A. H.
Velasquez, P.
Alemany, M. I.
Pena, P.
De Aza, P. N.
[J]. JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 2007, 90 (04) : 1200 - 1207
[10] De Aza AH, 2004, J AM CERAM SOC, V87, P449

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