Leucine-Rich Repeat-Containing G-Protein-Coupled Receptors as Markers of Adult Stem Cells

被引：289

作者：

Barker, Nick ^{[1
]}

Clevers, Hans

机构：

[1] Hubrecht Inst, NL-3584 CT Utrecht, Netherlands

来源：

GASTROENTEROLOGY | 2010年 / 138卷 / 05期

关键词：

Lgr5; Lgr6; Stem Cell Marker; GPCR; MOUSE SMALL-INTESTINE; HUMAN HEPATOCELLULAR CARCINOMAS; GLYCOPROTEIN HORMONE-RECEPTORS; COLORECTAL-CANCER CELLS; MALE REPRODUCTIVE-TRACT; BETA-CATENIN MUTATIONS; LABEL-RETAINING CELLS; HAIR FOLLICLE BULGE; EPITHELIAL-CELLS; SELF-RENEWAL;

D O I：

10.1053/j.gastro.2010.03.002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Molecular markers are used to characterize and track adult stem cells. Colon cancer research has led to the identification of 2 related receptors, leucine-rich repeat-containing, G-protein-coupled receptors (Lgr)5 and Lgr6, that are expressed by small populations of cells in a variety of adult organs. Genetic mouse models have allowed the visualization, isolation, and genetic marking of Lgr5(+ve) and Lgr6(+ve) cells and provided evidence that they are stem cells. The Lgr5(+ve) cells were found to occupy locations not commonly associated with stem cells in the stomach, small intestine, colon, and hair follicles. A multipotent population of skin stem cells express Lgr6. Single Lgr5(+ve) stem cells from the small intestine and the stomach can be cultured into long-lived organoids. Further studies of these markers might reveal adult stem cell populations in additional tissues. Identification of the ligands for Lgr5 and 6 will help elucidate stem cell functions and modes of intracellular signaling.

引用

页码：1681 / 1696

页数：16

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