Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) having a high hydroxyvalerate content with valeric acid feeding

被引：35

作者：

Khanna, Shilpi ^{[1
]}

Srivastava, Ashok K. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biochem Engn & Biotechnol, New Delhi 110016, India

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 34卷 / 06期

关键词：

valeric acid; P(3HB-co-3HV); propionic acid; Wautersia eutropha; PHB;

D O I：

10.1007/s10295-007-0207-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The capability of different organic acids to produce a derivative of PHB [poly(3-hydroxybutyric-co-3-hydroxyvaleric acid), P(3HB-co-3HV)] was examined in shake flask cultivations. Propionic and valeric acids demonstrated the potential to produce P(3HB-co-3HV) under nitrogen limiting conditions at 30 degrees C. The addition time and the initial concentration of valeric acid needed for a high cellular HV content were identified by extensive experimentation. Fed-batch cultivation in 7-1 bioreactor with valeric acid feeding resulted in the production of PHA containing 54% HV units.

引用

页码：457 / 461

页数：5

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