Automated Glycan Assembly of 19F-labeled Glycan Probes Enables High-Throughput NMR Studies of Protein-Glycan Interactions

Protein-glycan interactions mediate important biological processes, including pathogen host invasion and cellular communication. Herein, we showcase an expedite approach that integrates automated glycan assembly (AGA) of F-19-labeled probes and high-throughput NMR methods, enabling the study of protein-glycan interactions. Synthetic Lewis type 2 antigens were screened against seven glycan binding proteins (GBPs), including DC-SIGN and BambL, respectively involved in HIV-1 and lung infections in immunocompromised patients, confirming the preference for fucosylated glycans (Le(x), H type 2, Le(y)). Previously unknown glycan-lectin weak interactions were detected, and thermodynamic data were obtained. Enzymatic reactions were monitored in real-time, delivering kinetic parameters. These results demonstrate the utility of AGA combined with F-19 NMR for the discovery and characterization of glycan-protein interactions, opening up new perspectives for F-19-labeled complex glycans.