Celastrus paniculatus Willd. (Celastraceae) is an important medicinal tree valued for its seeds. Indiscriminate collection of this plant from the habitat zones has posed a serious threat to its existence in the wild, results in globally a threatened plant species. Ex situ conservation is a prioritized agenda of research for redlisted plant species. Semi-hardwood stem cuttings collected from 10 to 15 year old trees were treated with different concentrations (0, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 g l(-1)) of growth hormones; indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) for positive effect on root induction. After 90 days of treatment, results were analyzed for percentage of rooting, root number and average root length per rooted cuttings. The highest rooting (57%) response with highest number of roots (similar to 77.2) was obtained in indole-3-acetic acid at 3.0 g l(-1). Overall, high significant (P a parts per thousand currency sign 0.05) rooting response was noticed in IAA treatment comparative to hormones IBA and NAA. Rooted cuttings exhibited 100% survival in the experimental field. This study reveals the propagation of semi-hardwood cuttings derived from partially matured branches is possible and it provides a basis for conservation of this threatened plant species by reducing pressure on its natural populations.