HPLC/DAD analysis of phenolic compounds from lavender and its application to quality control

被引：45

作者：

Areias, FM ^{[1
]}

Valentao, P ^{[1
]}

Andrade, PB ^{[1
]}

Moreira, MM ^{[1
]}

Amaral, J ^{[1
]}

Seabra, RM ^{[1
]}

机构：

[1] Univ Porto, Fac Farm, CEQUP, Lab Farmacognosia, P-4050 Porto, Portugal

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 16期

关键词：

D O I：

10.1081/JLC-100100510

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed phase HPLC procedure is proposed for the determination of eight phenolic compounds (2-O-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin-7-O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS 2 (5 mu m particle size: 25.0 x 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid (19:1) and acetonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.

引用

页码：2563 / 2572

页数：10

共 9 条

[1] A previous study of phenolic profiles of quince, pear, and apple purees by HPLC diode array detection for the evaluation of quince puree genuineness
Andrade, PB
Carvalho, ARF
Seabra, RM
Ferreira, MA
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (03) : 968 - 972
[2] Simultaneous determination of flavonoids, phenolic acids, and coumarins in seven medicinal species by HPLC/diode-array detector
Andrade, PB
Seabra, RM
Valentao, P
Areias, F
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1998, 21 (18) : 2813 - 2820
[3] [Anonymous], TREASE EVANS PHARMAC
[4] BIOSYNTHESIS OF THE COUMARINS .4. THE FORMATION OF COUMARIN AND HERNIARIN IN LAVENDER
BROWN, SA
[J]. PHYTOCHEMISTRY, 1963, 2 (02) : 137 - 144
[5] BIOSYNTHESIS OF COUMARIN AND HERNIARIN IN LAVENDER
BROWN, SA
[J]. SCIENCE, 1962, 137 (3534) : 977 - &
[6] PARIS R, 1971, MATIERE MED, V3, P256
[7] VANHELLEMONT J, 1986, COMPENDIUM PHYTOTHER, P226
[8] VINCENZI M, 1991, FITOTERAPIA, V62, P39
[9] 1989, PHARMACOPEE FRANCAIS

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