Direct determination of sialic acids in serum by capillary electrophoresis with UV detection

被引：1

作者：

Dong, XL

Xu, Y

Lin, BC

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

[2] Dalian Third Municipal Hosp, Dalian 116033, Peoples R China

[3] Dalian Obstet & Gynecol Hosp, Dalian 116012, Peoples R China

来源：

CHROMATOGRAPHIA | 2000年 / 52卷 / 1-2期

关键词：

capillary electrophoresis; sialic acids; human serum;

D O I：

10.1007/BF02490785

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method for the determination of N-acetylneuraminic acid (NANA) and N-glycolylneuraminic acid (NGNA) has been developed using high-performance capillary electrophoresis with UV detection at 195 nm, without pre or post-column derivatisation. The acids were separated in a 50-cm, fused-silica capillary (50 mu m i.d, 45.5-cm effective length) with Na2B4O7-Na2HPO4 buffer. The detection limit for NANA is a concentration of 9.6 x 10(-6) M or, in terms of mass: 3.879 x 10(-14) mol (39 fmol). This method is applicable to determination of NANA in normal human serum. The results were also compared with those of the colorimetrie method.

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收藏

页码：14 / 16

页数：3

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