MiR-221 Exacerbate Cell Proliferation and Invasion by Targeting TIMP3 in Papillary Thyroid Carcinoma

被引：23
作者：
Diao, Yingbin [1 ]
Fu, Hongyu [1 ]
Wang, Qian [2 ]
机构：
[1] Daqing Oilfield Gen Hosp, Dept Endocrinol, Daqing, Heilongjiang, Peoples R China
[2] Daqing Oilfield Gen Hosp, Dept Rheumatol, Daqing, Heilongjiang, Peoples R China
来源：
AMERICAN JOURNAL OF THERAPEUTICS | 2017年 / 24卷 / 03期
关键词：
papillary thyroid cancer; miR-221; TIMP3; cell proliferation and invasion; EXPRESSION AFFECTS; TUMOR PROGRESSION; DOWN-REGULATION; CANCER; GROWTH; OVEREXPRESSION; TUMORIGENICITY; APOPTOSIS; MIGRATION; PTEN;
D O I：
10.1097/MJT.0000000000000420
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
MiR-221 is frequently upregulated in papillary thyroid cancer (PTC) tissues and cell lines, and this study was designed to validate the association of miR-221 with PTC proliferation, apoptosis, and migration. We observed that miR-221 suppressed TIMP3 expression by binding to 39 untranslated region of TIMP3 mRNA, and TIMP3 expression was increased with the presence of miR-221 inhibitors; TIMP3 siRNA could reverse the effects of miR-221 inhibitors on PTC cells. The results indicated that miR-221 exacerbated PTC by downregulating the expression of TIMP3. The effects of miR-221 and TIMP3 in vivo were also confirmed by human PTC-bearing mice models which suggest consistent results with those in vitro studies. In summary, miR-221 could aggravate cell proliferation and invasion of PTC by targeting TIMP3.
引用
收藏
页码：E317 / E328
页数：12
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