In vitro organogenesis of Passiflora alata

被引：28

作者：

Chiaverini Pinto, Ana Paula ^{[1
]}

Monteiro-Hara, Alessandra Cristina B. A. ^{[1
]}

Liborio Stipp, Liliane Cristina ^{[1
]}

Januzzi Mendes, Beatriz Madalena ^{[1
]}

机构：

[1] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, SP, Brazil

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2010年 / 46卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Adventitious bud; Ethylene; Silver nitrate; Sweet passion fruit; EDULIS-F FLAVICARPA; INORGANIC MEDIUM COMPONENTS; YELLOW PASSIONFRUIT; MICROPROPAGATION; EXPLANTS; RESISTANCE; CULTURES; DEGENER; GENE;

D O I：

10.1007/s11627-009-9251-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Passiflora alata in vitro organogenesis was studied based on explant type, culture medium composition, and incubation conditions. The results indicated that the morphogenic process occurred more efficiently when hypocotyl segment-derived explants were cultured in media supplemented with cytokinin and AgNO3 incubated under a 16-h photoperiod. The shoot bud elongation and plant development were obtained by transferring the material to MSM culture medium supplemented with GA(3) and incubated in flasks with vented lids. Histological analyses of the process revealed that the difficulties in obtaining plants could be related to the development of protuberances and leaf primordia structures, which did not contain shoot apical meristem. Roots developed easily by transferring elongated shoots to 1/2 MSM culture medium. Plant acclimatization occurred successfully, and somaclonal variation was not visually detected. The efficiency of this organogenesis protocol will be evaluated for genetic transformation of this species to obtain transgenic plants expressing genes that can influence the resistance to Cowpea aphid borne mosaic virus.

引用

页码：28 / 33

页数：6

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