Molecular surveillance of anti-malarial resistance pfcrt, pfmdr1, and pfk13 polymorphisms in African Plasmodium falciparum imported parasites to Wuhan, China

被引:17
作者
Cheng, Weijia [1 ]
Song, Xiaonan [1 ]
Tan, Huabing [2 ]
Wu, Kai [3 ]
Li, Jian [1 ,2 ]
机构
[1] Hubei Univ Med, Sch Basic Med Sci, Dept Human Parasitol, Shiyan 442000, Peoples R China
[2] Hubei Univ Med, Renmin Hosp, Dept Infect Dis, Shiyan 442000, Peoples R China
[3] Wuhan City Ctr Dis Prevent & Control, Dept Schistosomiasis & Endem Dis, Wuhan 430024, Peoples R China
基金
中国国家自然科学基金;
关键词
Imported malaria; Plasmodium falciparum; Anti-malarial resistance; Molecular surveillance; Wuhan; ARTEMISININ RESISTANCE; CHLOROQUINE-RESISTANCE; MULTIDRUG-RESISTANCE; PIPERAQUINE RESISTANCE; DRUG-RESISTANCE; BIOKO ISLAND; COPY NUMBER; MALARIA; MUTATIONS; GENE;
D O I
10.1186/s12936-021-03737-8
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background Imported malaria parasites with anti-malarial drug resistance (ADR) from Africa is a serious public health challenge in non-malarial regions, including Wuhan, China. It is crucial to assess the ADR status in African Plasmodium falciparum isolates from imported malaria cases, as this will provide valuable information for rational medication and malaria control. Methods During 2017-2019, a cross-sectional study was carried out in Wuhan, China. Peripheral blood 3 ml of returned migrant workers from Africa was collected. The target fragments from pfcrt, pfmdr1, and k13 propeller (pfk13) genes were amplified, sequenced, and analysed. Results In total, 106 samples were collected. Subsequently, 98.11% (104/106), 100% (106/106), and 86.79% (92/106) of these samples were successfully amplified and sequenced for the pfcrt (72-76), pfmdr1, and pfk13 genes, respectively. The prevalence of the pfcrt 76 T, pfmdr1 86Y, and pfmdr1 184F mutations was 9.62, 4.72, and 47.17%, respectively. At codons 72-76, the pfcrt locus displayed three haplotypes, CVMNK (wild-type), CVIET (mutation type), CV M/I N/E K/T (mixed type), with 87.50%, 9.62%, and 2.88% prevalence, respectively. For the pfmdr1 gene, NY (wild type), NF and YF (mutant type), N Y/F, Y Y/F, and N/Y Y/F (mixed type) accounted for 34.91, 43.40, 3.77, 15.09, 0.94, and 1.89% of the haplotypes, respectively. A total of 83 isolates with six unique haplotypes were found in pfcrt and pfmdr1 combined haplotypes, of which NY-CVMNK and NF-CVMNK accounted for 40.96% (34/83) and 43.37% (36/83), respectively. Furthermore, 90 cases were successfully sequenced (84.91%, 90/106) at loci 93, 97, 101, and 145, and 78 cases were successfully sequenced (73.58%, 78/106) at loci 343, 353, and 356 for pfcrt. However, the mutation was observed only in locus 356 with 6.41%. For pfk13, mutations reported in Southeast Asia (at loci 474, 476, 493, 508, 527, 533, 537, 539, 543, 553, 568, 574, 578, and 580) and Africa (at loci 550, 561, 575, 579, and 589) were not observed. Conclusions The present data from pfcrt and pfmdr1 demonstrate that anti-malarial drugs including chloroquine, amodiaquine, and mefloquine, remain effective against malaria treatment in Africa. The new mutations in pfcrt related to piperaquine resistance remain at relatively low levels. Another source of concern is the artemether-lumefantrine resistance-related profiles of N86 and 184F of pfmdr1. Although no mutation in pfk13 is detected, molecular surveillance must continue.
引用
收藏
页数:8
相关论文
共 51 条
[21]   The Plasmodium vivax in China: decreased in local cases but increased imported cases from Southeast Asia and Africa [J].
Feng, Jun ;
Xiao, Huihui ;
Zhang, Li ;
Yan, He ;
Feng, Xinyu ;
Fang, Wen ;
Xia, Zhigui .
SCIENTIFIC REPORTS, 2015, 5
[22]   Prevalence of mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, and association with ex vivo susceptibility to common anti-malarial drugs against African Plasmodium falciparum isolates [J].
Foguim, Francis Tsombeng ;
Bogreau, Herve ;
Gendrot, Mathieu ;
Mosnier, Joel ;
Fonta, Isabelle ;
Benoit, Nicolas ;
Amalvict, Remy ;
Madamet, Marylin ;
Wein, Sharon ;
Pradines, Bruno .
MALARIA JOURNAL, 2020, 19 (01)
[23]   In-Vivo Efficacy of Chloroquine to Clear Asymptomatic Infections in Mozambican Adults: A Randomized, Placebo-controlled Trial with Implications for Elimination Strategies [J].
Galatas, Beatriz ;
Nhamussua, Lidia ;
Candrinho, Baltazar ;
Mabote, Lurdes ;
Cistero, Pau ;
Gupta, Himanshu ;
Rabinovich, Regina ;
Menendez, Clara ;
Macete, Eusebio ;
Saute, Francisco ;
Mayor, Alfredo ;
Alonso, Pedro ;
Bassat, Quique ;
Aide, Pedro .
SCIENTIFIC REPORTS, 2017, 7
[24]   Plasmodium falciparum isolates from Angola show the StctVMNT haplotype in the pfcrt gene [J].
Gama, Bianca E. ;
Pereira-Carvalho, Guilhermina A. L. ;
Lutucuta Kosi, Florbela J. I. ;
Almeida de Oliveira, Natalia K. ;
Fortes, Filomeno ;
Rosenthal, Philip J. ;
Daniel-Ribeiro, Claudio T. ;
Ferreira-da-Cruz, Maria de Fatima .
MALARIA JOURNAL, 2010, 9
[25]   Identification of large variation in pfcrt, pfmdr-1 and pfubp-1 markers in Plasmodium falciparum isolates from Ethiopia and Tanzania [J].
Golassa, Lemu ;
Kamugisha, Erasmus ;
Ishengoma, Deus S. ;
Baraka, Vito ;
Shayo, Alex ;
Baliraine, Frederick N. ;
Enweji, Nizar ;
Erko, Berhanu ;
Aseffa, Abraham ;
Choy, Angel ;
Swedberg, Gote .
MALARIA JOURNAL, 2015, 14
[26]   Effect of mass dihydroartemisinin-piperaquine administration in southern Mozambique on the carriage of molecular markers of antimalarial resistance [J].
Gupta, Himanshu ;
Galatas, Beatriz ;
Chidimatembue, Arlindo ;
Huijben, Silvie ;
Cistero, Pau ;
Matambisso, Gloria ;
Nhamussua, Lidia ;
Simone, Wilson ;
Bassat, Quique ;
Menard, Didier ;
Ringwald, Pascal ;
Rabinovich, N. Regina ;
Alonso, Pedro L. ;
Saute, Francisco ;
Aide, Pedro ;
Mayor, Alfredo .
PLOS ONE, 2020, 15 (10)
[27]   Drug-Resistant Polymorphisms and Copy Numbers in Plasmodium falciparum, Mozambique, 2015 [J].
Gupta, Himanshu ;
Macete, Eusebio ;
Bulo, Helder ;
Salvador, Crizolgo ;
Warsame, Marian ;
Carvalho, Eva ;
Menard, Didier ;
Ringwald, Pascal ;
Bassat, Quique ;
Enosse, Sonia ;
Mayor, Alfredo .
EMERGING INFECTIOUS DISEASES, 2018, 24 (01) :40-48
[28]   Evolution and expansion of multidrug-resistant malaria in southeast Asia: a genomic epidemiology study [J].
Hamilton, William L. ;
Amato, Roberto ;
van der Pluijm, Rob W. ;
Jacob, Christopher G. ;
Huynh Hong Quang ;
Nguyen Thanh Thuy-Nhien ;
Tran Tinh Hien ;
Hongvanthong, Bouasy ;
Chindavongsa, Keobouphaphone ;
Mayxay, Mayfong ;
Huy, Rekol ;
Leang, Rithea ;
Huch, Cheah ;
Dysoley, Lek ;
Amaratunga, Chanaki ;
Suon, Seila ;
Fairhurst, Rick M. ;
Tripura, Rupam ;
Peto, Thomas J. ;
Sovann, Yok ;
Jittamala, Podjanee ;
Hanboonkunupakarn, Borimas ;
Pukrittayakamee, Sasithon ;
Nguyen Hoang Chau ;
Imwong, Mallika ;
Dhorda, Mehul ;
Vongpromek, Ranitha ;
Chan, Xin Hui S. ;
Maude, Richard J. ;
Pearson, Richard D. ;
Nguyen, T. ;
Rockett, Kirk ;
Drury, Eleanor ;
Goncalves, Sonia ;
White, Nicholas J. ;
Day, Nicholas P. ;
Kwiatkowski, Dominic P. ;
Dondorp, Arjen M. ;
Miotto, Olivo .
LANCET INFECTIOUS DISEASES, 2019, 19 (09) :943-951
[29]   Selection of Plasmodium falciparum Multidrug Resistance Gene 1 Alleles in Asexual Stages and Gametocytes by Artemether-Lumefantrine in Nigerian Children with Uncomplicated Falciparum Malaria [J].
Happi, C. T. ;
Gbotosho, G. O. ;
Folarin, O. A. ;
Sowunmi, A. ;
Hudson, T. ;
O'Neil, M. ;
Milhous, W. ;
Wirth, D. F. ;
Oduola, A. M. J. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (03) :888-895
[30]   Spread of Artemisinin Resistance in Malaria [J].
Hawkes, Michael ;
Conroy, Andrea L. ;
Kain, Kevin C. .
NEW ENGLAND JOURNAL OF MEDICINE, 2014, 371 (20) :1944-1945