Comparison between enzyme-linked immunosorbent assay, indirect fluorescent antibody and rapid conglutination tests in detecting antibodies against Babesia bovis

被引:30
作者
Araujo, FR
Madruga, CR
Leal, CRB
Schenk, MAM
Kessler, RH
Marques, APC
Lemaire, DC
机构
[1] Univ Desenvolvimento Estado & da Regiao do Pantan, BR-79037280 Campo Grande, MS, Brazil
[2] EMBRAPA, CNPGC, BR-79002970 Campo Grande, Brazil
[3] Univ Fed Bahia, Inst Ciencias Saude, Salvador, BA, Brazil
关键词
Babesia bovis; conglutination; ELISA; hemoparasite; indirect fluorescent antibody test; serological tests; Bahia; Brazil; cattle; piroplasmida;
D O I
10.1016/S0304-4017(97)00171-4
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The performance of an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFAT) and a rapid conglutination test (RCT) for the detection of antibodies against Babesia bovis, was evaluated with 462 cattle sera from Bahia State, Brazil, The results showed a concordance of 96.6% between the ELISA and IFAT, 90.5% between the ELISA and RCT, and 91.8% between the IFAT and RCT. Although the prevalence rates determined by ELISA (97.2%) and IFAT (96.8%) were higher than that indicated by the RCT (92.9%), performances of the three serological tests were very similar and characterized the region studied as enzootically stable to B.bovis. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:101 / 108
页数:8
相关论文
共 22 条
[1]  
Barci L. A. G., 1994, Revista Brasileira de Parasitologia Veterinaria, V3, P79
[2]   A MICROPLATE ENZYME-IMMUNOASSAY FOR DETECTING AND MEASURING ANTIBODIES TO BABESIA-BOVIS IN CATTLE SERUM [J].
BARRY, DN ;
RODWELL, BJ ;
TIMMS, P ;
MCGREGOR, W .
AUSTRALIAN VETERINARY JOURNAL, 1982, 59 (05) :136-140
[3]  
BESSENGER R, 1983, ONDERSTEPOORT J VET, V50, P115
[4]  
Blandino T, 1994, Arch Med Res, V25, P219
[5]   AN IMPROVED ELISA FOR THE DETECTION OF ANTIBODIES AGAINST BABESIA-BOVIS USING EITHER A NATIVE OR A RECOMBINANT BABESIA-BOVIS ANTIGEN [J].
BOSE, R ;
JACOBSON, RH ;
GALE, KR ;
WALTISBUHL, DJ ;
WRIGHT, IG .
PARASITOLOGY RESEARCH, 1990, 76 (08) :648-652
[6]   CURRENT STATE AND FUTURE-TRENDS IN THE DIAGNOSIS OF BABESIOSIS [J].
BOSE, R ;
JORGENSEN, WK ;
DALGLIESH, RJ ;
FRIEDHOFF, KT ;
DEVOS, AJ .
VETERINARY PARASITOLOGY, 1995, 57 (1-3) :61-74
[7]  
Chieves L., 1989, P341
[8]  
*CTR PANAM ZOON RA, 1979, PROC EST PREV MUESTR
[9]  
*I BRAS GEOGR EST, 1991, PESQ PEC MUN
[10]  
KESSLER RH, 1987, PESQUI AGROPECU BRAS, V22, P747