miR-5195-3p Inhibits Proliferation and Invasion of Human Bladder Cancer Cells by Directly Targeting Oncogene KLF5

被引:49
作者
Jiang, Zhangjie [1 ]
Zhang, Yida [2 ]
Cao, Runfu [2 ]
Li, Li [3 ]
Zhong, Kezhao [2 ]
Chen, Qingsheng [2 ]
Xiao, Jianjun [2 ]
机构
[1] Nanchang Univ, Affiliated Hosp 1, Dept Anesthesiol, Nanchang, Jiangxi, Peoples R China
[2] Nanchang Univ, Affiliated Hosp 1, Dept Urol, 17 Yongwai St, Nanchang 330000, Jiangxi, Peoples R China
[3] Ganzhou Renmin Hosp, Dept Anesthesiol, Ganzhou, Peoples R China
关键词
Bladder cancer; miR-5195-3p; Cell proliferation; Cell invasion; Kriippel-like factor 5 (KLF5); KRUPPEL-LIKE FACTORS; TRANSCRIPTION FACTORS; APOPTOSIS;
D O I
10.3727/096504016X14831120463349
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
miRNAs play a key role in the carcinogenesis of many cancers, including bladder cancer. In the current study, the role of miR-5195-3p, a quite recently discovered and poorly studied miRNA, in the proliferation and invasion of human bladder cancer cells was investigated. Our data displayed that, compared with healthy volunteers (control) and SU-HUC-1 normal human bladder epithelial cells, miR-5195-3p was sharply down regulated in bladder cancer patients and five human bladder cancer cell lines. The oligo miR-5195-3p mimic or miR-5195-3p antagomir was subsequently transfected into both T24 and BIU-87 bladder cancer cell lines. The miR-5195-3p mimic robustly increased the miR-5195-3p expression level and distinctly reduced the proliferation and invasion of T24 and BIU-87 cells. In contrast, the miR-5195-3p antagomir had an opposite effect on miR-5195-3p expression, cell proliferation, and invasion. Our data from bioinformatic and luciferase reporter gene assays identified that miR-5195-3p targeted the mRNA 3'-UTR of Kruppel-like factor 5 (KLF5), which is a proven proto-oncogene in bladder cancer. miR-5195-3p sharply reduced KLF5 expression and suppressed the expression or activation of its several downstream genes that are kinases improving cell survival or promoting cell cycle regulators, including ERK1/2, VEGFA, and cyclin D1. In conclusion, miR-5195-3p suppressed proliferation and invasion of human bladder cancer cells via suppression of KLF5.
引用
收藏
页码:1081 / 1087
页数:7
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