Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production

被引:29
作者
Camargo, L. E. A. [1 ]
Pedroso, L. S. [1 ]
Vendrame, S. C. [2 ]
Mainardes, R. M. [2 ]
Khalil, N. M. [2 ]
机构
[1] Fac Guairaca, Dept Pharm, Rua 15 Novembro 7050, BR-85010000 Guarapuava, PR, Brazil
[2] Univ Estadual Ctr Oeste UNICTR, Lab Pharmaceut Nanotechnol, Rua Simeao Camargo Varela de Sa 03, BR-85040080 Guarapuava, PR, Brazil
关键词
teas; fermentation method; Candida spp; phenolic compounds; reactive oxygen species; OXIDATIVE STRESS; BLACK TEA; GREEN TEA; MYELOPEROXIDASE; SUSCEPTIBILITY; POLYPHENOLS; INHIBITION; TOXICITY; EXTRACTS; SERUM;
D O I
10.1590/1519-6984.18814
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The antioxidant and anticandidal activities of leaves obtained from Camellia sinensis by non-fermentation (green and white teas), semi-fermentation (red tea) and fermentation method (black tea) were investigated. It was evaluated the total phenolic content by Folin-Ciocalteau assay; antioxidant capacities were evaluated in vitro using DPPH and ABTS radicals, hypochlorous acid and superoxide anion scavenger assays, induced hemolysis, lipid peroxidation by conjugated diene formation and myeloperoxidase activity. Anticandidal activity was performed on three strains of Candida spp. The results showed that non-fermented teas have a higher concentration of phenolic compounds, and then presented the best inhibitory activity of AAPH-induced hemolysis, the best inhibition of conjugated diene formation and more pronounced antioxidant activity in all tests. The highest anticandidal activity was obtained from fermented tea, followed by non-fermented tea. These results indicate that the antioxidant activity demonstrated has no direct relation with the anticandidal activity.
引用
收藏
页码:428 / 434
页数:7
相关论文
共 47 条
[1]   Antioxidant and antimicrobial activities of tea infusions [J].
Almajano, M. Pilar ;
Carbo, Rosa ;
Jimenez, J. Angel Lopez ;
Gordon, Michael H. .
FOOD CHEMISTRY, 2008, 108 (01) :55-63
[2]   Human myeloperoxidase in innate and acquired immunity [J].
Arnhold, Juergen ;
Flemmig, Joerg .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2010, 500 (01) :92-106
[3]   Oxidative Stress in Laparoscopic Versus Open Abdominal Surgery: A Systematic Review [J].
Arsalani-Zadeh, Reza ;
Ullah, Sana ;
Khan, Shakeeb ;
MacFie, John .
JOURNAL OF SURGICAL RESEARCH, 2011, 169 (01) :E59-E68
[4]   Distribution and biosynthesis of flavan-3-ols in Camellia sinensis seedlings and expression of genes encoding biosynthetic enzymes [J].
Ashihara, Hiroshi ;
Deng, Wei-Wei ;
Mullen, William ;
Crozier, Alan .
PHYTOCHEMISTRY, 2010, 71 (5-6) :559-566
[5]   Comparison of the antioxidant capacity and the antimicrobial activity of black and green tea [J].
Bancirova, Martina .
FOOD RESEARCH INTERNATIONAL, 2010, 43 (05) :1379-1382
[6]   Oxidative stress: Relations between the formation of reactive species and the organism's defense. [J].
Barreiros, ALBS ;
David, JM ;
David, JP .
QUIMICA NOVA, 2006, 29 (01) :113-123
[7]  
BORA K., 2005, REV VISAO ACAD, V6, P6
[8]   Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia [J].
Chan, E. W. C. ;
Lim, Y. Y. ;
Chew, Y. L. .
FOOD CHEMISTRY, 2007, 102 (04) :1214-1222
[9]   Distinct HDL subclasses present similar intrinsic susceptibility to oxidation by HOCl [J].
Chantepie, Sandrine ;
Malle, Ernst ;
Sattler, Wolfgang ;
Chapman, M. John ;
Kontush, Anatol .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2009, 487 (01) :28-35
[10]  
CLSI. National Committee for Clinical Laboratory Standards, 2002, M27A2 CLSI