Studies on a microfluidic chip based on continuous flow PCR amplification system

被引：0

作者：

Liu, JH ^{[1
]}

Yin, XF ^{[1
]}

Xu, GM ^{[1
]}

Fang, ZL ^{[1
]}

Chen, HZ ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem, Inst Microanal Syst, Hangzhou 310028, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2003年 / 24卷 / 02期

关键词：

PCR amplification; microfluidic chip; DNA;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A PCR amplification system comprising a syringe pump sample processor, a microfluidic PCR chip and a 3-temperature-zone heating block was developed in this work. The system is capable of performing continuous PCR amplification with a low carryover between neighboring samples. The substrate thickness and thermal insulation of the temperature control unit were optimized. A 24-cycle PCR amplification of a 500-base pair fragment from lambda-DNA was successfully performed with a total reaction time of 4. 9 min.

引用

页码：232 / 235

页数：4

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