The isolation of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose from Acer truncatum Bunge by high-speed counter-current chromatography

被引：42
作者：
Zhao, Wen-Hua
Gao, Chun-Chun
Ma, Xiao-Feng
Bai, Xiao-Yu
Zhang, Ying-Xia [1 ]
机构：
[1] Capital Med Univ, Dept Biol Chem, Beijing 100069, Peoples R China
[2] Harbin Commercial Univ, Dept Pharm, Harbin 150076, Peoples R China
[3] Grad Univ, Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China
[4] Capital Med Univ, Lab Liver Protect & Regulat, Beijing 100069, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 850卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
1,2,3,4,6-penta-O-galloyl-beta-D-glucose; Acer truncatum Bunge; counter-current chromatography;
D O I：
10.1016/j.jchromb.2006.11.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose from the ethyl acetate extract of the leaves of Acer truncatum Bunge using a two-phase system composed of n-hexane-ethyl acetate-methanol-water at a volume ratio of (0.25:5:1:5, v/v/v/v) for the first time. Each injection of 80 mg crude extract yielded 7.25 mg of pure 1,2,3,4,6-penta-O-galloyl-beta-D-glucose. High-performance liquid chromatography (HPLC) analyses of the CCC fraction revealed that the purity of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose was over 95%. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：523 / 527
页数：5
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