Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus

被引:10
作者
Puckette, Michael [1 ]
Primavera, Victoria [2 ,3 ]
Martel, Erica [4 ]
Barrera, Jose [2 ,3 ]
Hurtle, William [1 ]
Clark, Benjamin [4 ]
Kamicker, Barbara [2 ,3 ]
Zurita, Mariceny [2 ,3 ]
Brake, David [5 ]
Neilan, John [1 ]
机构
[1] US Dept Homeland Secur Sci & Technol Directorate, Plum Isl Anim Dis Ctr, New York, NY 11944 USA
[2] Leidos, Plum Isl Anim Dis Ctr, New York, NY 11944 USA
[3] SAIC, Plum Isl Anim Dis Ctr, New York, NY 11944 USA
[4] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr Res Participat Program, New York, NY 11944 USA
[5] BioQuest Associates LLC, POB 787, Stowe, VT 05672 USA
来源
VIRUSES-BASEL | 2022年 / 14卷 / 05期
关键词
virus-like particle; foot-and-mouth disease; foot-and-mouth disease virus; vaccine; swine; cattle; platform; mammalian cell culture; plasmid; DIVA; PROTECT GUINEA-PIGS; CAPSID PROTEINS; FMDV; EXPRESSION; SEROTYPES; EFFICACY;
D O I
10.3390/v14050989
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
RNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vaccine platforms. In this report, we describe a plasmid-based virus-like particle (VLP) production platform utilizing transiently transfected mammalian cell cultures that combines both the rapid response adaptability of nucleic-acid-based vaccines with the ability to produce intact capsid epitopes required for immunity. Formulated vaccines which employed this platform conferred complete protection from clinical foot-and-mouth disease in both swine and cattle. This novel platform can be quickly adapted to new viral strains and serotypes through targeted exchanges of only the FMDV capsid polypeptide nucleic acid sequences, from which processed structural capsid proteins are derived. This platform obviates the need for high biocontainment manufacturing facilities to produce inactivated whole-virus vaccines from infected mammalian cell cultures, which requires upstream expansion and downstream concentration of large quantities of live virulent viruses.
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页数:11
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